Time-resolved enzymatic determination of glucose using a fluorescent europium probe for hydrogen peroxide

DOI to cite this document:: 10.5283/epub.20370

Wu, Meng ; Lin, Zhihong ; Dürkop, Axel ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 04 Apr 2011 10:51

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Abstract

An enzymatic assay for glucose based on the use of the fluorescent probe for hydrogen peroxide, europium(III) tetracycline (EuTc), is described. The weakly fluorescent EuTc and enzymatically generated H2O2 form a strongly fluorescent complex (EuTc-H2O2) whose fluorescence decay profile is significantly different. Since the decay time of EuTc-H2O2 is in the microseconds time domain, fluorescence ...