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Time-resolved enzymatic determination of glucose using a fluorescent europium probe for hydrogen peroxide

DOI to cite this document:
Wu, Meng ; Lin, Zhihong ; Dürkop, Axel ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 04 Apr 2011 10:51


An enzymatic assay for glucose based on the use of the fluorescent probe for hydrogen peroxide, europium(III) tetracycline (EuTc), is described. The weakly fluorescent EuTc and enzymatically generated H2O2 form a strongly fluorescent complex (EuTc–H2O2) whose fluorescence decay profile is significantly different. Since the decay time of EuTc–H2O2 is in the microseconds time domain, fluorescence ...


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