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Fluorescent Imaging of Citrate and Other Intermediates in the Citric Acid Cycle

Lin, Zhihong ; Wu, Meng ; Schäferling, M. ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 06 Apr 2011 09:16


Abstract

Getting the picture: Citrate and other intermediates of the citric acid cycle can be imaged by time-resolved fluorescence with a Eu3+ tetracycline complex as a fluorescent probe (see picture). The time resolution enables discrimination between intermediates, and enzymatic conversions are not needed.


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