URN zum Zitieren dieses Dokuments: urn:nbn:de:bvb:355-epub-204249
Hessing, M., van Schijndel, H. B., van Grunsven, W. M., Wolf, Hans J. und Middeldorp, J. M.
(1992)
Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells.
Journal of chromatography 599 (1-2), S. 267-272.
Zum PubMed-Eintrag dieses Artikels
Zusammenfassung
Truncated Epstein-Barr virus (EBV) membrane antigen gp350/220 (EBV-MA) lacking the membrane anchor was expressed and secreted into the medium of recombinant Chinese hamster ovary cells that had been cultured in Plasmapur hollow-fibre modules using defined serum-free medium. The EBV-MA in the medium was concentrated by 70% (w/v) ammonium sulphate precipitation and subsequently purified by ...
Zusammenfassung
Truncated Epstein-Barr virus (EBV) membrane antigen gp350/220 (EBV-MA) lacking the membrane anchor was expressed and secreted into the medium of recombinant Chinese hamster ovary cells that had been cultured in Plasmapur hollow-fibre modules using defined serum-free medium. The EBV-MA in the medium was concentrated by 70% (w/v) ammonium sulphate precipitation and subsequently purified by immunoaffinity chromatography using an anti-EBV-MA (EBV.0T6) monoclonal antibody (mAb) column. Adsorbed antigen was eluted with 3 M MgCl2 in phosphate-buffered saline, concentrated by Mono Q anion-exchange chromatography and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, silver staining and Western blotting using EBV-positive serum and anti-EBV-MA specific mAbs. Monospecific polyclonal rabbit antibodies against the purified EBV-MA were raised and purified by protein G affinity chromatography. For the measurement of EBV-MA antigen levels a sandwich enzyme-linked immunosorbent assay using rabbit polyclonal antibodies and a horseradish peroxidase-conjugated anti-MA mAb was developed having a detection level of 10 ng/ml.
Bibliographische Daten exportieren
Dokumentenart: | Artikel |
---|
Datum: | 1992 |
---|
Institutionen: | Medizin > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
---|
Identifikationsnummer: | |
---|
Klassifikation: | Notation | Art |
---|
Animals | MESH | Antigens, Viral/isolation & purification | MESH | Blotting, Western | MESH | CHO Cells | MESH | Cricetinae | MESH | Electrophoresis, Polyacrylamide Gel | MESH | Enzyme-Linked Immunosorbent Assay | MESH | Herpesvirus 4, Human/immunology | MESH | Recombinant Proteins/isolation & purification | MESH | Viral Envelope Proteins/isolation & purification | MESH | Viral Matrix Proteins | MESH |
|
---|
Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
---|
Status: | Veröffentlicht |
---|
Begutachtet: | Unbekannt / Keine Angabe |
---|
An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
---|
Eingebracht am: | 11 Apr 2011 10:53 |
---|
Zuletzt geändert: | 08 Mrz 2017 08:29 |
---|
Dokumenten-ID: | 20424 |
---|