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Recombinant antigens in viral diagnosis

Vornhagen, R. ; Baur, A. ; Jahn, G. ; Hinderer, W. ; Nebel-Schickel, H. ; Horn, J. ; Wolf, Hans J. ; Sonneborn, H. H.



Abstract

DNA-fragments coding for a variety of different viral antigens have been cloned and expressed in E. coli. Selected purified recombinant antigens were used for detection of specific antibodies by the means of ELISA technique. This approach has been used for the development of four different ELISA's for the detection of HIV- and EBV-specific antibodies.


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