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Homogeneous Luminescence Decay Time-Based Assay Using Energy Transfer from Nanospheres

Kürner, Jens M. ; Wolfbeis, Otto S. ; Klimant, Ingo
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Date of publication of this fulltext: 15 Apr 2011 09:16


Abstract

Following a study on the feasibility of resonance energy transfer (RET) from carboxylated nanospheres with an incorporated phosphorescent donor to a cationic polyelectrolyte/acceptor aggregate on their surface, a novel scheme for homogeneous assays is presented that is based on RET from phosphorescent biotinylated nanospheres to fluorescently labeled streptavidin (SA). The phosphorescent ...

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