Capacitive Approach To Determine Phospholipase A2 Activity toward Artificial and Natural Substrates

DOI to cite this document:: 10.5283/epub.20456

Mirsky, Vladimir M. ; Mass, Markus ; Krause, Christian ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Apr 2011 13:14

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Abstract

A capacitive approach has been employed to develop a novel method to determine phospholipase activity. The sensing electrodes have a structure like Au/S(CH2)17CH3/substrate/electrolyte. Hydrolysis of the substrate, mediated by phospholipase A2, leads to the formation of water-soluble products from the insoluble substrate. This results in desorption of these products into aqueous phase and ...