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Serum Chloride Optical Sensors Based on Dynamic Quenching of the Fluorescence of Photo-Immobilized Lucigenin
Huber, Christian, Krause, Christian, Werner, Tobias and Wolfbeis, Otto S.
(2003)
Serum Chloride Optical Sensors Based on Dynamic Quenching of the Fluorescence of Photo-Immobilized Lucigenin.
Microchimica Acta 142 (4), pp. 245-253.
Date of publication of this fulltext: 18 Apr 2011 13:18
Article
DOI to cite this document: 10.5283/epub.20472
Abstract
This article describes the preparation and performance of an optical sensor for continuous measurement of chloride at extracellular (serum) levels (20-200 mM). The sensor is based on dynamic quenching of the fluorescence of lucigenin which was photo-immobilized on a hydrogel. Quenching occurs via a collisional mechanism, and the decrease in fluorescence intensity on exposure to 100 mM chloride ...
This article describes the preparation and performance of an optical sensor for continuous measurement of chloride at extracellular (serum) levels (20-200 mM). The sensor is based on dynamic quenching of the fluorescence of lucigenin which was photo-immobilized on a hydrogel. Quenching occurs via a collisional mechanism, and the decrease in fluorescence intensity on exposure to 100 mM chloride typically is -60%. It allows the determination of chloride in the 1 to 200 mM concentration range, with a precision of +/-3 mM at 120 mM. Bromide, iodide and salicylate act as interferents, while the effect of pH and oxygen is negligibly small. The sensor displays strong fluorescence intensity, excellent reproducibility, long-term stability, response times in the order of 2-5 min, and is used in a commercial serum and blood chloride analyzer.
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Details
| Item type | Article | ||||
| Journal or Publication Title | Microchimica Acta | ||||
| Publisher: | SPRINGER-VERLAG WIEN | ||||
|---|---|---|---|---|---|
| Place of Publication: | VIENNA | ||||
| Volume: | 142 | ||||
| Number of Issue or Book Chapter: | 4 | ||||
| Page Range: | pp. 245-253 | ||||
| Date | 2003 | ||||
| Institutions | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik | ||||
| Identification Number |
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| Keywords | INDICATORS; COEXTRACTION; MEMBRANE; chloride sensor; fluorescence; quenching; lucigenin; photo-immobilization | ||||
| Dewey Decimal Classification | 500 Science > 540 Chemistry & allied sciences | ||||
| Status | Published | ||||
| Refereed | Unknown | ||||
| Created at the University of Regensburg | Unknown | ||||
| Item ID | 20472 |
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