Serum Chloride Optical Sensors Based on Dynamic Quenching of the Fluorescence of Photo-Immobilized Lucigenin

DOI to cite this document:: 10.5283/epub.20472

Huber, Christian ; Krause, Christian ; Werner, Tobias ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Apr 2011 13:18

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Abstract

This article describes the preparation and performance of an optical sensor for continuous measurement of chloride at extracellular (serum) levels (20–200 mM). The sensor is based on dynamic quenching of the fluorescence of lucigenin which was photo-immobilized on a hydrogel. Quenching occurs via a collisional mechanism, and the decrease in fluorescence intensity on exposure to 100mM chloride ...