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Sandwich nucleic acid hybridization: a method with a universally usable labeled probe for various specific tests

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Wolf, Hans J. ; Leser, Ulrike ; Haus, M. ; Gu, S. Y. ; Pathmanathan, R.
Date of publication of this fulltext: 20 Apr 2011 12:43


Nucleic acid hybridization is widely used for scientific applications but essentially restricted to specialized laboratories. The use of recombinant m 13 phages as hybridization probes (Hu and Messing (1980) Gene 17, 271; Messing (1983) Methods Enzymol. 101, 20) offers a considerable advantage over the commonly used recombinant plasmids as the preparation of the DNA probe is very simple and it ...


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