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Expression of the Epstein-Barr virus 138-kDa early protein in Escherichia coli for the use as antigen in diagnostic tests

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Motz, M. ; Fan, J. ; Seibl, R. ; Jilg, Wolfgang ; Wolf, Hans J.
Date of publication of this fulltext: 20 Apr 2011 12:37


We have attempted to produce the 138-kDa early protein (ep 138) of Epstein-Barr virus (EBV) in Escherichia coli. This protein was found, by immunoprecipitation, to be a clinically relevant antigen, especially for the determination of the IgA-titer in patients with nasopharyngeal carcinoma (NPC). Since the expression of the entire ep 138 coding region was unsuccessful, we synthesized only the ...


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