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Time-Resolved Luminescence Imaging of Hydrogen Peroxide Using Sensor Membranes in a Microwell Format

Schäferling, Michael ; Wu, Meng ; Enderlein, Jörg ; Bauer, Henrik ; Wolfbeis, Otto S.


Abstract

We demonstrate an optical imaging scheme for hydrogen peroxide in a microwell-based format using the europium(III) tetracycline complex as the fluorescent probe, which is incorporated into a polyacrylonitrile-co-polyacrylamide polymer matrix. The resulting sensor membranes are integrated into a 96-microwell plate. Hydrogen peroxide can be visualized by means of time-resolved luminescence lifetime ...

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