Engineered Bacteria Based Biosensors for Monitoring Bioavailable Heavy Metals

DOI to cite this document:: 10.5283/epub.20731

Leth, Suzanne ; Maltoni, Susanna ; Simkus, Remigijus ; Mattiasson, Bo ; Corbisier, Philippe ; Klimant, Ingo ; Wolfbeis, Otto S.

; Csöregi, Elisabeth

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Date of publication of this fulltext: 13 May 2011 08:34

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Abstract

This work presents an integrated analytical system based on immobilized engineered microorganisms and bioluminescence measurements for monitoring of bioavailable heavy metal ions (Cu being chosen as a model ion). A strain of microorganisms from Alcaligenes eutrophus (AE1239) was genetically engineered by inserting a luxCDABE operon from Vibrio fischeri under the control of a copper-induced ...