Time-Resolved Luminescence Energy Transfer Immunobinding Study Using a Ruthenium–Ligand Complex as a Donor Label

DOI to cite this document:: 10.5283/epub.20748

Augustin, Christine M. ; Oswald, Bernhard ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 13 May 2011 08:27

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Abstract

A novel immunosystem is described that exploits the effect of luminescence energy transfer from a luminescently labeled antigen to a fluorescent antibody. A luminescent ruthenium-ligand complex (D-455) with absorption/emission maxima at 456/639 nm, respectively, was employed as the donor label, and a squaraine-type cyanine label (636/655 nm), as the fluorescent acceptor label. Specifically, the ...