Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem

DOI to cite this document:: 10.5283/epub.20750

Oswald, Bernhard ; Lehmann, Frank ; Simon, Lydia ; Terpetschnig, Ewald ; Wolfbeis, Otto S.

PDF
(97kB) - Repository staff only

Date of publication of this fulltext: 13 May 2011 08:47

Alternative links to fulltext:DOI

Details

Indexed in

Export bibliographical data

Abstract

We describe two near-infrared fluorescent squaraine dyes (Sq635 and Sq660), their spectra, their covalent linkage to proteins, and their use as donor and acceptor, respectively, in a fluorescence resonance energy transfer (FRET) immunoassay based on the use of red lasers. The dyes show quantum yields of around 10% in the free form and up to 68% when bound to proteins. If converted into their ...