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Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem

Oswald, Bernhard, Lehmann, Frank, Simon, Lydia, Terpetschnig, Ewald and Wolfbeis, Otto S. (2000) Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem. Analytical Biochemistry 280 (2), pp. 272-277.

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Date of publication of this fulltext: 13 May 2011 08:47

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We describe two near-infrared fluorescent squaraine dyes (Sq635 and Sq660), their spectra, their covalent linkage to proteins, and their use as donor and acceptor, respectively, in a fluorescence resonance energy transfer (FRET) immunoassay based on the use of red lasers. The dyes show quantum yields of around 10% in the free form and up to 68% when bound to proteins. If converted into their ...


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Item type:Article
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
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Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Created at the University of Regensburg:Unknown
Item ID:20750
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