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Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem

Oswald, Bernhard ; Lehmann, Frank ; Simon, Lydia ; Terpetschnig, Ewald ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 13 May 2011 08:47


Abstract

We describe two near-infrared fluorescent squaraine dyes (Sq635 and Sq660), their spectra, their covalent linkage to proteins, and their use as donor and acceptor, respectively, in a fluorescence resonance energy transfer (FRET) immunoassay based on the use of red lasers. The dyes show quantum yields of around 10% in the free form and up to 68% when bound to proteins. If converted into their ...

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