Wolf, Hans J., zur Hausen, H., Klein, G., Becker, V., Henle, G. and Henle, W.
(1975)
Attempts to detect virus-specific DNA sequences in human tumors. III. Epstein-Barr viral DNA in non-lymphoid nasopharyngeal carcinoma cells.
Medical microbiology and immunology 161 (1), pp. 15-21.
Date of publication of this fulltext: 05 May 2011 06:35at PubMed
Abstract
Fourteen tumors of the nasopharyngeal region were analyzed for the presence of Epstein-Barr virus-specific DNA by DNA-cRNA hybridization. These data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against EBV-related antigens. With one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with ...
Abstract
Fourteen tumors of the nasopharyngeal region were analyzed for the presence of Epstein-Barr virus-specific DNA by DNA-cRNA hybridization. These data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against EBV-related antigens. With one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with EBV-cRNA. Tumors of predominantly epithelial morphology annealed in the highest range. In situ-hybridization of freeze sections from a tumor containing almost equal amounts of tumor cells and lymphocytes revealed hybridizing DNA within nuclei of non-lymphoid cells. Although these data do not exclude the presence of EBV-DNA within lymphoid cells, they clearly demonstrate that in nasopharyngeal carcinomas the vast majority of EBV-specific DNA rests within non-lymphoid cells.
Export bibliographical data
| Item type: | Article |
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| Date: | 1975 |
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| Institutions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
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| Identification Number: | |
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| Classification: | | Notation | Type |
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| Carcinoma/microbiology | MESH | | Carcinoma, Squamous Cell/microbiology | MESH | | DNA, Neoplasm/isolation & purification | MESH | | DNA, Viral/isolation & purification | MESH | | Herpesvirus 4, Human/isolation & purification | MESH | | Humans | MESH | | Hybridization, Genetic | MESH | | Nasopharyngeal Neoplasms/pathology | MESH | | Nucleic Acid Hybridization | MESH | | RNA, Neoplasm/isolation & purification | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Unknown |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 20772 |
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