Polyaniline-coated microtiter plates for use in longwave optical bioassays

DOI to cite this document:: 10.5283/epub.21066

Piletsky, Sergey A. ; Panasyuk, Tatiana L. ; Piletskaya, Elena V. ; Sergeeva, Tanya A. ; El’skaya, Anna V. ; Pringsheim, Erika ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 06 Jun 2011 13:01

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Abstract

A technique for coating the wells of microtiterplates with polyaniline layers and with polyaniline/enzyme layers is presented. The resulting wells are shown to be useful for assaying enzyme substrates (as exemplified for glucose via pH) and hydrogen peroxide (via the redox properties of the film). Analyte detection is based on monitoring the absorption spectra of the polyaniline, which turn ...