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Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA

Wu, Meng ; Lin, Zhihong ; Mirsky, Vladimir ; Wolfbeis, Otto S. Zhou, Yikai , eds.


In this research, a surface-plasmon resonance biosensor for nucleic acid detection was constructed, optimized and characterized. The kinetic response on hybridization was also studied. The biosensor was use as a platform for the study of the surface coverage control by single-strand or double-strand oligonucleotide immobilization. The dsDNA immobilization and offers a new way to surface coverage control for the nucleic acid biosensor construction.

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