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Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA

Wu, Meng, Lin, Zhihong, Mirsky, Vladimir and Wolfbeis, Otto S. (2001) Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA. In: Zhou, Yikai, (ed.) Proceedings / International Conference on Sensor Technology (ISTC 2001): 10-12 October 2001, Wuhan, China. Proc. SPIE, 4414. SPIE, the International Society for Optical Engineering, Bellingham, Wash., pp. 23-26. ISBN 0-8194-4119-8.

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Abstract

In this research, a surface-plasmon resonance biosensor for nucleic acid detection was constructed, optimized and characterized. The kinetic response on hybridization was also studied. The biosensor was use as a platform for the study of the surface coverage control by single-strand or double-strand oligonucleotide immobilization. The dsDNA immobilization and offers a new way to surface coverage control for the nucleic acid biosensor construction.


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Item type:Book section
Date:2001
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1117/12.440180DOI
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:21101
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