Dummer, R., Becker, J. C., Eilles, Christoph, Schäfer, E., Börner, W. and Burg, G.
(1993)
T cells migrate to tumour sites after extracorporeal interleukin 2 stimulation and reinfusion in a patient with metastatic melanoma.
The British journal of dermatology 128 (4), pp. 399-403.
Date of publication of this fulltext: 10 Jun 2011 07:48at PubMed
Abstract
Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes ...
Abstract
Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes (CD3+). After radiolabelling with indium-111, the cells were reinfused. Gamma-camera imaging revealed an enrichment at the tumour sites. Immunostaining of tumour tissue taken before and after scintigraphy demonstrated CD25+ T lymphocytes (CD2+, CD3+), but no natural killer cells (CD16+, CD56+) infiltrating the metastases. LAK cell enrichment at melanoma metastases in vivo did not involve natural killer cells, but was characterized by increased numbers of activated T lymphocytes in this patient.
Export bibliographical data
| Item type: | Article |
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| Date: | 1993 |
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| Institutions: | Medicine > Abteilung für Nuklearmedizin |
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| Identification Number: | | Value | Type |
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| 8494752 | PubMed ID |
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| Classification: | | Notation | Type |
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| Cell Movement | MESH | | Cytotoxicity, Immunologic | MESH | | Humans | MESH | | Interleukin-2/pharmacology | MESH | | Killer Cells, Lymphokine-Activated/transplantation | MESH | | Killer Cells, Natural/immunology | MESH | | Leukocyte Count | MESH | | Lymphocyte Activation | MESH | | Male | MESH | | Melanoma/pathology | MESH | | Middle Aged | MESH | | Receptors, Interleukin-2/immunology | MESH | | Recombinant Proteins/pharmacology | MESH | | Skin Neoplasms/secondary | MESH | | T-Lymphocyte Subsets/immunology | MESH | | Up-Regulation | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 21111 |
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