T cells migrate to tumour sites after extracorporeal interleukin 2 stimulation and reinfusion in a patient with metastatic melanoma

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urn:nbn:de:bvb:355-epub-211115 Copy

Dummer, R. ; Becker, J. C. ; Eilles, Christoph ; Schäfer, E. ; Börner, W. ; Burg, G.

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Date of publication of this fulltext: 10 Jun 2011 07:48

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Details

Item type:	Article
Journal or Publication Title:	The British journal of dermatology
Publisher:	Wiley-Blackwell
Volume:	128
Number of Issue or Book Chapter:	4
Page Range:	pp. 399-403
Date:	1993
Institutions:	Medicine > Abteilung für Nuklearmedizin
Identification Number:	Value Type 8494752 PubMed ID
Classification:	Notation Type Cell Movement MESH Cytotoxicity, Immunologic MESH Humans MESH Interleukin-2/pharmacology MESH Killer Cells, Lymphokine-Activated/transplantation MESH Killer Cells, Natural/immunology MESH Leukocyte Count MESH Lymphocyte Activation MESH Male MESH Melanoma/pathology MESH Middle Aged MESH Receptors, Interleukin-2/immunology MESH Recombinant Proteins/pharmacology MESH Skin Neoplasms/secondary MESH T-Lymphocyte Subsets/immunology MESH Up-Regulation MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Unknown
Item ID:	21111

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Abstract

Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes ...

Abstract

Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes (CD3+). After radiolabelling with indium-111, the cells were reinfused. Gamma-camera imaging revealed an enrichment at the tumour sites. Immunostaining of tumour tissue taken before and after scintigraphy demonstrated CD25+ T lymphocytes (CD2+, CD3+), but no natural killer cells (CD16+, CD56+) infiltrating the metastases. LAK cell enrichment at melanoma metastases in vivo did not involve natural killer cells, but was characterized by increased numbers of activated T lymphocytes in this patient.

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Metadata last modified: 03 Jun 2018 03:58

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