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Separation of a serum-derived tumoricidal factor from a helper factor for plaque-forming cells

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Männel, Daniela N. ; Farrar, J. J. ; Mergenhagen, S. E.
Date of publication of this fulltext: 20 Jun 2011 06:21


C3H/HeN mice administered BCG followed by lipopolysaccharide 14 days later released into their serum a cytotoxic factor for tumor cells and a factor that restored the anti-SRBC plaque-forming cell response of nude mouse spleen cells (helper activity). Gel filtration of serum containing the cytotoxic and the helper activities indicated that both factors exhibited an apparent m.w. of 125,000 to ...


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