Antigen-specific electrophoretic cell separation for immunological investigations

URN to cite this document:: urn:nbn:de:bvb:355-epub-219815
DOI to cite this document:: 10.5283/epub.21981

Hansen, Ernil ; Wustrow, T. P. ; Hannig, K.

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Date of publication of this fulltext: 02 Sep 2011 09:21

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Abstract

Preincubation of human blood lymphocytes with cell surface antigen specific antibodies under non-capping conditions reduces the electrophoretic mobility of the corresponding lymphocyte subpopulation. Antigen-positive and antigen-negative cells can be separated by free flow electrophoresis with high yield, purity and viability. The use of fluorescence-labelled second antibodies augments the ...