Zeiller, K. und Hansen, Ernil
(1978)
Characterization of rat bone marrow lymphoid cells. I. A study of the distribution parameters of sedimentation velocity, volume and electrophoretic mobility.
The journal of histochemistry and cytochemistry 26 (5), S. 369-381.
Veröffentlichungsdatum dieses Volltextes: 05 Sep 2011 13:30Zum PubMed-Eintrag dieses Artikels
Zusammenfassung
Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells. Up to 4.5 mm/hr five different populations with discrete distributions in volume (coefficient of variation 10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10%) ...
Zusammenfassung
Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells. Up to 4.5 mm/hr five different populations with discrete distributions in volume (coefficient of variation 10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10%) were observed. Three of the small sized populations represented lymphocytes and small normoblasts and two of the larger sized populations represented myeloid cells. Almost all of these cells were in the G0/G1 cycle phase. In the faster sedimenting fractions which contained immature myeloid, erythroid and undefined blast cells and two S phase populations, discrete volume distributions were not evaluated. The cell populations with homogeneous volume (particularly the small lymphocytes) showed high density variations which condiserably impair the separation resolution. The cells sedimenting slower than 3.5 mm/hr were further separated by means of free flow electrophoresis into three peaks differing in electrophoretic mobility (EPM). The peaks of low and high EPM contained two populations and the peak of medium EPM contained three populations all characterized by normal volume distributions of uniform coefficient of variation between 11% and 14%. The small cells in the peaks of high and medium EPM were normolblasts and the other cells were lymphocytes. The biological significance of these results is discussed.
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| Dokumentenart: | Artikel |
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| Datum: | 1978 |
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| Institutionen: | Medizin > Lehrstuhl für Anästhesiologie |
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| Identifikationsnummer: | |
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| Klassifikation: | | Notation | Art |
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| Animals | MESH | | Bone Marrow/metabolism | MESH | | Bone Marrow Cells | MESH | | Cell Count | MESH | | Cell Cycle | MESH | | Cell Division | MESH | | Cell Separation | MESH | | Electrophoresis | MESH | | Lymphocytes/cytology | MESH | | Rats | MESH | | Statistics as Topic | MESH | | Thymidine/metabolism | MESH | | Ultracentrifugation | MESH |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
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| Dokumenten-ID: | 21999 |
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