Characterization of rat bone marrow lymphoid cells. I. A study of the distribution parameters of sedimentation velocity, volume and electrophoretic mobility

URN to cite this document:

urn:nbn:de:bvb:355-epub-219990 Copy

DOI to cite this document:

10.5283/epub.21999 Copy

Zeiller, K. ; Hansen, Ernil

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Date of publication of this fulltext: 05 Sep 2011 13:30

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Details

Item type:	Article
Journal or Publication Title:	The journal of histochemistry and cytochemistry
Publisher:	The Histochemistry Society; Sage
Volume:	26
Number of Issue or Book Chapter:	5
Page Range:	pp. 369-381
Date:	1978
Institutions:	Medicine > Lehrstuhl für Anästhesiologie
Identification Number:	Value Type 659838 PubMed ID
Classification:	Notation Type Animals MESH Bone Marrow/metabolism MESH Bone Marrow Cells MESH Cell Count MESH Cell Cycle MESH Cell Division MESH Cell Separation MESH Electrophoresis MESH Lymphocytes/cytology MESH Rats MESH Statistics as Topic MESH Thymidine/metabolism MESH Ultracentrifugation MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Unknown
Item ID:	21999

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Abstract

Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells. Up to 4.5 mm/hr five different populations with discrete distributions in volume (coefficient of variation 10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10%) ...

Abstract

Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells. Up to 4.5 mm/hr five different populations with discrete distributions in volume (coefficient of variation 10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10%) were observed. Three of the small sized populations represented lymphocytes and small normoblasts and two of the larger sized populations represented myeloid cells. Almost all of these cells were in the G0/G1 cycle phase. In the faster sedimenting fractions which contained immature myeloid, erythroid and undefined blast cells and two S phase populations, discrete volume distributions were not evaluated. The cell populations with homogeneous volume (particularly the small lymphocytes) showed high density variations which condiserably impair the separation resolution. The cells sedimenting slower than 3.5 mm/hr were further separated by means of free flow electrophoresis into three peaks differing in electrophoretic mobility (EPM). The peaks of low and high EPM contained two populations and the peak of medium EPM contained three populations all characterized by normal volume distributions of uniform coefficient of variation between 11% and 14%. The small cells in the peaks of high and medium EPM were normolblasts and the other cells were lymphocytes. The biological significance of these results is discussed.

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