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Cell wall regeneration by Nicotiana tabacum protoplast: Chemical and biochemical aspects

Blaschek, W. ; Haaß, D. ; Koehler, H. ; Franz, Gerhard


Protoplasts isolated enzymatically from the mesophyll of tobacco leaves (Nicotiana tabacum L.) were cultured in a defined liquid medium. The composition of the cell wall polysaccharides (CWP) and of the extracellular polysaccharides (ECP) synthesized after 1–4 days of culture was investigated by gas-liquid chromatography (GLC) in comparison with that derived from tobacco mesophyll and tobacco ...


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