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Gas-liquid chromatography-mass spectrometry analysis of anticoagulant active curdlan sulfates

Alban, S. ; Franz, Gerhard



Abstract

1. To evaluate the sulfation pattern of anticoagulant active curdlan sulfates, a methylation method, based on the synthesis of alkylated alditol acetates, followed by GLC-MS examination, was developed. 2. By the specific cleavage of the sulfate esters, using solvolysis and the substitution of these liberated OH groups with ethyl iodide, a third kind of substitution was introduced in the alkylated ...

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