Evidence for tyrosine-linked glycosaminoglycan in a bacterial surface protein

URN to cite this document:: urn:nbn:de:bvb:355-epub-228532
DOI to cite this document:: 10.5283/epub.22853

Peters, J. ; Rudolf, S. ; Oschkinat, H. ; Mengele, R. ; Sumper, Manfred ; Kellermann, J. ; Lottspeich, F. ; Baumeister, W.

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Date of publication of this fulltext: 05 Dec 2011 06:55

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Abstract

The S-layer protein of Acetogenium kivui was subjected to proteolysis with different proteases and several high molecular mass glycosaminoglycan peptides containing glucose, galactosamine and an unidentified sugar-related component were separated by molecular sieve chromatography and reversed-phase HPLC and subjected to N-terminal sequence analysis. By methylation analysis glucose was found to be ...