Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose. Purification and identification of sulfated dolichyl monophosphoryl tetrasaccharides from halobacteria

URN to cite this document:

urn:nbn:de:bvb:355-epub-228856 Copy

DOI to cite this document:

10.5283/epub.22885 Copy

Lechner, J. ; Wieland, F. ; Sumper, Manfred

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Date of publication of this fulltext: 08 Dec 2011 07:07

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Details

Item type:	Article
Journal or Publication Title:	The Journal of biological chemistry
Publisher:	American society for biochemistry and molecular biology
Volume:	260
Number of Issue or Book Chapter:	2
Page Range:	pp. 860-866
Date:	1985
Institutions:	Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Alumni or Retired > Prof. Dr. Manfred Sumper
Identification Number:	Value Type 2857171 PubMed ID
Classification:	Notation Type Asparagine MESH Carbohydrate Conformation MESH Chromatography, High Pressure Liquid MESH Dolichol Phosphates/analysis MESH Glucose MESH Halobacterium/metabolism MESH Lipids/analysis MESH Magnetic Resonance Spectroscopy MESH Polyisoprenyl Phosphates/analysis MESH Polysaccharides/isolation & purification MESH
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Unknown
Item ID:	22885

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Abstract

A novel type of N-glycosidic linkage, asparaginyl glucose, occurs in the cell surface glycoprotein of halobacteria (Wieland, F., Heitzer, R., and Schaefer, W. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 5470-5474). Sulfated oligosaccharides containing glucuronic acids are attached to the polypeptide chain via this linkage. Here we describe the isolation and chemical characterization of lipid-linked ...

Abstract

A novel type of N-glycosidic linkage, asparaginyl glucose, occurs in the cell surface glycoprotein of halobacteria (Wieland, F., Heitzer, R., and Schaefer, W. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 5470-5474). Sulfated oligosaccharides containing glucuronic acids are attached to the polypeptide chain via this linkage. Here we describe the isolation and chemical characterization of lipid-linked precursors of these saccharides, and these have the following new features. Rather than the bacterial undecaprenol, a C60-dolichol is the carrier lipid. The oligosaccharide is bound to this lipid via a monophosphate, rather than a pyrophosphate bridge. Sulfation of the saccharides is completed while they are linked to lipid and does not occur after transfer of the saccharides to protein.

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Metadata last modified: 26 Nov 2020 05:44

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