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Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase

URN to cite this document:
urn:nbn:de:bvb:355-epub-229267
Sumper, Manfred ; Luce, R.
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Date of publication of this fulltext: 13 Dec 2011 07:06


Abstract

Highly purified coliphage Qbeta replicase when incubated without added template synthesizes self-replicating RNA species in an autocatalytic reaction. In this paper we offer strong evidence that this RNA production is directed by templates generated de novo during the lag phase. Contamination of the enzyme by traces of RNA templates was ruled out by the following experimental results: (1) ...

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