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G-protein alpha-subunits in cytosolic and membranous fractions of human neutrophils

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Rudolph, U. ; Koesling, D. ; Hinsch, K. D. ; Seifert, Roland ; Bigalke, M. ; Schultz, Günter ; Rosenthal, W.
Date of publication of this fulltext: 24 Jan 2012 11:52


In plasma membranes of human neutrophils, we identified two major pertussis toxin substrates of 40 kDa Mr with pI values of 5.30 and 5.37. Only the acidic of the two substrates was also present in neutrophil cytosol. Two-dimensional tryptic peptide maps revealed a high degree of homology of cytosolic and particulate substrates. Purified G-protein beta gamma-complex stimulated pertussis ...


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