The role of nucleoside-diphosphate kinase reactions in G protein activation of NADPH oxidase by guanine and adenine nucleotides

URN to cite this document:: urn:nbn:de:bvb:355-epub-232672
DOI to cite this document:: 10.5283/epub.23267

Seifert, Roland ; Rosenthal, W. ; Schultz, Günter ; Wieland, T. ; Gierschick, P. ; Jakobs, K. H.

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Date of publication of this fulltext: 26 Jan 2012 08:34

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Abstract

NADPH-oxidase-catalyzed superoxide (O2-) formation in membranes of HL-60 leukemic cells was activated by arachidonic acid in the presence of Mg2+ and HL-60 cytosol. The GTP analogues, guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S] and guanosine 5'-[beta,gamma-imido]triphosphate, being potent activators of guanine-nucleotide-binding proteins (G proteins), stimulated O2- formation up to ...