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Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression.

URN to cite this document:
urn:nbn:de:bvb:355-epub-237532
Fath, Stephan ; Bauer, Asli Petra ; Liss, Michael ; Spriestersbach, Anne ; Maertens, Barbara ; Hahn, Peter ; Ludwig, Christine ; Schäfer, Frank ; Graf, Marcus ; Wagner, Ralf
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Date of publication of this fulltext: 11 Apr 2012 09:02


Abstract

Autologous expression of recombinant human proteins in human cells for biomedical research and product development is often hampered by low expression yields limiting subsequent structural and functional analyses. Following RNA and codon optimization, 50 candidate genes representing five classes of human proteins--transcription factors, ribosomal and polymerase subunits, protein kinases, membrane ...

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