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Biooxidation of n-hexanol by alcohol oxidase and catalase in biphasic and micellar systems without solvent

Karra-Chaabouni, M. ; Meziani, A. ; Thomas, D. ; Touraud, Didier ; Kunz, Werner



Abstract

Alcohol oxidase from Pichia pastoris together with catalase from bovine liver was used to oxidize n-hexanol to hexanal. For this purpose, an aqueous buffer solution was mixed with large amounts of hexanol by simple agitation, yielding a biphasic system, or by adding the nonionic surfactant Brij 35. Initial velocities and reaction yields after 24 h were measured as a function of various ...

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