Role of water activity on the synthesis of geranyl butyrate by a Mucor miehei esterase in a solvent-free system

Abstract

The initial activity rate for the synthesis of geranyl butyrate by an esterase from Mucor miehei increased with increasing water activity and decreased above 0.5 water activity. The conversion yield after 75 h was about 75% for different, but low, initial water activities and decreased above 0.5 water activity. This behavior is related to water-enzyme interactions.