Abstract
Composite scaffolds of homogeneously mixed esterified hyaluronan (HY) and gelatin (G) were manufd. with variable component compns. (HY100%; HY95%/G5%; HY70%/G30%). The goals of this study were to analyze the produced composite scaffolds using phys. and chem. methods, for example, SEM, IR-spectroscopy, water contact angle, protein assay, and tensile testing as well as to assess the effects of ...
Abstract
Composite scaffolds of homogeneously mixed esterified hyaluronan (HY) and gelatin (G) were manufd. with variable component compns. (HY100%; HY95%/G5%; HY70%/G30%). The goals of this study were to analyze the produced composite scaffolds using phys. and chem. methods, for example, SEM, IR-spectroscopy, water contact angle, protein assay, and tensile testing as well as to assess the effects of adding gelatin to the composite scaffolds on attachment, proliferation, and chondrogenic differentiation of human mesenchymal stem cells. Nos. of attached cells were significantly higher on the composite material compared to pure hyaluronan at different time points of two-dimensional or three-dimensional cell culture (p< 0.02). In composite scaffolds, a significantly greater amt. of cartilage-specific extracellular matrix components was deposited after 28 days in culture (glycosaminoglycan: p < 0.001; collagen: p < 0.001) as compared with 100% hyaluronan scaffolds. Addnl., gelatin-contg. composite scaffolds displayed stronger promotion of collagen type II expression than pure hyaluronan scaffolds. The mechanism, based on which gelatin influences cell adhesion, was examd. The effect was inhibited by collagenase treatment of the composites or by addn. of α5β1-integrin blocking antibodies to the cell suspension. In summary, the results describe the establishment of a class of composite polymer scaffolds, consisting of esterified hyaluronan and gelatin, which are potentially useful for cell-based tissue engineering approaches using mesenchymal stem cells for chondrogenic differentiation. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009.
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