Scholz, H., Götz, K. H., Hamann, M. and Kurtz, Armin
(1994)
Differential effects of extracellular anions on renin secretion from isolated perfused rat kidneys.
The American journal of physiology. Renal Physiology 267 (6 Pt 2), F1076-F1081.
Date of publication of this fulltext: 04 Dec 2012 13:34at PubMed
Abstract
We investigated the relevance of anions for the regulation of renin secretion from the kidneys. For this purpose we measured renin release from isolated rat kidneys that were perfused with medium containing either 120 mmol/l (normal) chloride or 95 mmol/l of isethionate, acetate, or nitrate anions in exchange for equimolar amounts of chloride. Lowering the extracellular chloride concentration by ...
Abstract
We investigated the relevance of anions for the regulation of renin secretion from the kidneys. For this purpose we measured renin release from isolated rat kidneys that were perfused with medium containing either 120 mmol/l (normal) chloride or 95 mmol/l of isethionate, acetate, or nitrate anions in exchange for equimolar amounts of chloride. Lowering the extracellular chloride concentration by either of these maneuvers significantly enhanced renin secretion rates (RSR) at a perfusion pressure of 100 mmHg. Increasing pressure above 100 mmHg inhibited renin release in the presence of isethionate and acetate but not with nitrate anions. The renin stimulatory effects of isethionate and acetate but not that of nitrate anions disappeared in the presence of bumetanide (100 mumol/l), an inhibitor of macula densa chloride transport. Activation of renin secretion by isethionate and acetate was blunted with 100 pmol/l angiotensin II (ANG II), whereas tenfold higher concentrations of ANG II were required to attenuate the effect of nitrate ions. The amount of renin released in the presence of nitrate was fully additive to RSR values obtained with maximally effective doses of isoproterenol. These findings are consistent with the idea that impermeant anions such as isethionate and acetate enhance renin secretion from the kidneys predominantly via the tubular macula densa mechanism. The stimulatory influence of membrane-permeable nitrate anions appears to involve additional pathways and is mediated by a decreased calcium sensitivity of the renin secretory process rather than resulting from an adenosine 3',5'-cyclic monophosphate-dependent action.
Export bibliographical data
| Item type: | Article |
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| Date: | 1994 |
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| Institutions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
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| Identification Number: | | Value | Type |
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| 7810695 | PubMed ID |
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| Classification: | | Notation | Type |
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| Acetates/pharmacology | MESH | | Angiotensin II/pharmacology | MESH | | Animals | MESH | | Anions/pharmacology | MESH | | Blood Pressure | MESH | | Bumetanide/pharmacology | MESH | | Calcium/pharmacology | MESH | | Cell Membrane Permeability | MESH | | Chlorides/pharmacology | MESH | | Cyclic AMP/metabolism | MESH | | Extracellular Space | MESH | | Isethionic Acid/pharmacology | MESH | | Isoproterenol/pharmacology | MESH | | Kidney/secretion | MESH | | Kidney Tubules/secretion | MESH | | Male | MESH | | Nitrates/pharmacology | MESH | | Perfusion | MESH | | Rats | MESH | | Rats, Sprague-Dawley | MESH | | Renin/secretion | MESH |
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| Dewey Decimal Classification: | 500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 26919 |
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