Renin mRNA quantification using polymerase chain reaction in cultured juxtaglomerular cells. Short-term effects of cAMP on renin mRNA and secretion

URN to cite this document:: urn:nbn:de:bvb:355-epub-269304
DOI to cite this document:: 10.5283/epub.26930

Della Bruna, R. ; Kurtz, Armin ; Corvol, P. ; Pinet, F.

Preview

PDF
(8MB)

Date of publication of this fulltext: 11 Dec 2012 08:48

Alternative links to fulltext:Pubmed

Details

Preview

Export bibliographical data

Abstract

The aim of the present study was to set up a method to quantify renin mRNA levels in mouse renal juxtaglomerular cells, the main physiological site of renin synthesis. Because of the scarcity of the cells, a quantitative polymerase chain reaction had to be developed to measure renin mRNA. Juxtaglomerular cells were isolated and cultured for 2 days under various conditions, and renin mRNA was ...