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Renin mRNA quantification using polymerase chain reaction in cultured juxtaglomerular cells. Short-term effects of cAMP on renin mRNA and secretion

Della Bruna, R., Kurtz, Armin, Corvol, P. and Pinet, F. (1993) Renin mRNA quantification using polymerase chain reaction in cultured juxtaglomerular cells. Short-term effects of cAMP on renin mRNA and secretion. Circulation research 73 (4), pp. 639-648.

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Abstract

The aim of the present study was to set up a method to quantify renin mRNA levels in mouse renal juxtaglomerular cells, the main physiological site of renin synthesis. Because of the scarcity of the cells, a quantitative polymerase chain reaction had to be developed to measure renin mRNA. Juxtaglomerular cells were isolated and cultured for 2 days under various conditions, and renin mRNA was ...

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Item type:Article
Date:1993
Institutions:Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz
Identification Number:
ValueType
7690309PubMed ID
Classification:
NotationType
1-Methyl-3-isobutylxanthine/pharmacologyMESH
AnimalsMESH
Base SequenceMESH
Cells, CulturedMESH
Cyclic AMP/physiologyMESH
Forskolin/pharmacologyMESH
Juxtaglomerular Apparatus/metabolismMESH
MiceMESH
Mice, Inbred C57BLMESH
Molecular Sequence DataMESH
Polymerase Chain ReactionMESH
RNA, Messenger/geneticsMESH
Renin/secretionMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Item ID:26930
Owner only: item control page

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