Oxygen-dependent expression of the erythropoietin gene in rat hepatocytes in vitro

URN to cite this document:: urn:nbn:de:bvb:355-epub-269361
DOI to cite this document:: 10.5283/epub.26936

Eckardt, Kai-Uwe ; Pugh, C. W. ; Ratcliffe, P. J. ; Kurtz, Armin

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Date of publication of this fulltext: 05 Dec 2012 10:17

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Abstract

Since in juvenile rats the liver is the predominant site of erythropoietin (EPO) gene expression, we have used primary cultures of juvenile rat hepatocytes to establish and in vitro system for investigation of oxygen-dependent EPO formation. When isolated hepatocytes were incubated at reduced oxygen tensions for 18-48 h, we found increased secretion of EPO protein and elevated levels of EPO mRNA, ...