Insulin-like growth factors decrease oxygen-regulated erythropoietin production by human hepatoma cells (Hep G2)

URN to cite this document:

urn:nbn:de:bvb:355-epub-269468 Copy

Scholz, H. ; Baier, W. ; Ratcliffe, P. ; Eckardt, K. ; Zapf, J. ; Kurtz, Armin ; Bauer, C.

Preview

PDF (1MB)

Date of publication of this fulltext: 04 Dec 2012 13:52

---

Alternative links to fulltext:Pubmed

---

Details

Item type:	Article
Journal or Publication Title:	The American journal of physiology. Cell pyhsiology
Publisher:	American Physiological Society (APS)
Volume:	263
Number of Issue or Book Chapter:	2 Pt 1
Page Range:	C474-C479
Date:	1992
Institutions:	Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz
Identification Number:	Value Type 1325119 PubMed ID
Classification:	Notation Type Animals MESH Culture Media MESH Dose-Response Relationship, Drug MESH Erythropoietin/biosynthesis MESH Humans MESH Insulin/pharmacology MESH Insulin-Like Growth Factor I/pharmacology MESH Insulin-Like Growth Factor II/pharmacology MESH Liver Neoplasms/pathology MESH Liver Neoplasms, Experimental/pathology MESH Oxygen/pharmacology MESH Receptor, Insulin/metabolism MESH Receptors, Cell Surface/metabolism MESH Receptors, Somatomedin MESH Somatomedins/pharmacology MESH Tumor Cells, Cultured MESH
Dewey Decimal Classification:	500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Unknown
Item ID:	26946

Preview

Export bibliographical data

ASCII CitationAtomBibTeXData Cite XMLDataCite XML OAIDublin CoreEndNoteHTML CitationMETSMODSOpenAPCRDF+N-TriplesRDF+N3RDF+XMLReferReference ManagerSimple MetadataXMLxMetaDissPlus

---

Abstract

We examined the effects of insulin-like growth factors (IGFs) and insulin on erythropoietin (EPO) production by human hepatoma cells (Hep G2). Compared with normoxia (20% O2), EPO production by Hep G2 cells during a 72-h incubation was stimulated fivefold by exposure to low oxygen tension (1% O2) and nearly threefold by exposure to cobalt chloride (100 microM). IGF-I caused a ...

Abstract

We examined the effects of insulin-like growth factors (IGFs) and insulin on erythropoietin (EPO) production by human hepatoma cells (Hep G2). Compared with normoxia (20% O2), EPO production by Hep G2 cells during a 72-h incubation was stimulated fivefold by exposure to low oxygen tension (1% O2) and nearly threefold by exposure to cobalt chloride (100 microM). IGF-I caused a concentration-dependent attenuation of EPO formation under normoxic conditions and inhibited (maximally 50%) EPO production stimulated by either low oxygen tension or cobalt [half-maximal effect (ED50) approximately 5 nM]. The increase of EPO mRNA levels in response to hypoxia was significantly reduced by IGF-I. Similarly to IGF-I, IGF-II (ED50 approximately 8 nM) and insulin (ED50 approximately 80 nM) also inhibited EPO formation in Hep G2 cells. IGF-I (100 pM-100 nM) stimulated the incorporation of radiolabeled alanine as a measure for total protein synthesis, 3H-labeled thymidine incorporation into DNA, and glycogen synthesis at 20 and 1% O2 in a concentration-dependent fashion. IGF-I exhibited a high affinity for the IGF-I receptor (apparent Kd approximately 3 nM). Unlabeled insulin was greater than 100-fold less potent than IGF-I in competing for 125I-IGF-I binding (apparent Kd approximately 360 nM). Conversely, insulin bound to the insulin receptor with high affinity (apparent Kd approximately 0.3 nM), whereas IGF-I was less than 1% as potent in competing for 125I-insulin binding. In summary, IGFs and insulin exert a negative control function on oxygen-regulated EPO production in Hep G2 cells. The inhibitory effect of IGFs and insulin on EPO formation appears to be mediated via the IGF-I receptor.

---

---

Metadata last modified: 02 Jun 2018 19:28

Owner only: item control page

Download Statistics

Download Statistics

Download Statistics

Download Statistics

Downloads

Downloads per month over past year

Altmetric

Altmetric

Altmetric

Alternative Statistics (altmetrics)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)