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Pfeilschifter, J. ; Kurtz, Armin ; Bauer, Christian

Role of phospholipase C and protein kinase C in vasoconstrictor-induced prostaglandin synthesis in cultured rat renal mesangial cells

Pfeilschifter, J., Kurtz, Armin and Bauer, Christian (1986) Role of phospholipase C and protein kinase C in vasoconstrictor-induced prostaglandin synthesis in cultured rat renal mesangial cells. The Biochemical journal 234 (1), pp. 125-130.

Date of publication of this fulltext: 08 Jan 2013 08:11
Article
DOI to cite this document: 10.5283/epub.27009


Abstract

It was the aim of the present study to find out if a common mechanism exists by which the vasoconstrictive hormones angiotension II, noradrenaline and 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) increase prostaglandin E2 (PGE2) synthesis in cultures of rat renal mesangial cells. Angiotension II, noradrenaline and AGEPC stimulated PGE2 synthesis and uptake of 45Ca2+ in cultured ...

It was the aim of the present study to find out if a common mechanism exists by which the vasoconstrictive hormones angiotension II, noradrenaline and 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) increase prostaglandin E2 (PGE2) synthesis in cultures of rat renal mesangial cells. Angiotension II, noradrenaline and AGEPC stimulated PGE2 synthesis and uptake of 45Ca2+ in cultured mesangial cells. Both of these effects could be completely suppressed by the calcium channel blocker verapamil. Angiotensin II, noradrenaline and AGEPC caused a rapid breakdown of phosphatidylinositol 4,5-bisphosphate with a concomitant increase of 1,2-diacylglycerol and inositol trisphosphate, indicating an activation of phospholipase C by these hormones. Addition of verapamil had no effect on the hormone-induced stimulation of phospholipase C. The synthetic analogue of diacylglycerol, 1-oleoyl-2-acetylglycerol, and the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), both of which are known to stimulate protein kinase C, enhanced PGE2 synthesis. Chelation of extracellular calcium with EDTA or addition of verapamil abolished the effect of 1-oleoyl-2-acetylglycerol and phorbol ester on PGE2 synthesis. 1-Oleoyl-2-acetylglycerol and phorbol ester increased the uptake of 45Ca2+ by the cells in a dose-dependent manner and this effect could be blocked by verapamil. The entirety of these data leads us to suggest that vasoconstrictor-evoked synthesis of PGE2 in rat mesangial cells is mediated by the subsequent activation of phospholipase C and protein kinase C. The activation of protein kinase C by diacylglycerol is likely to be involved in the increase of the calcium permeability of the plasma membrane which is a prerequisite for PGE2 synthesis induced by vasoconstrictive hormones.



Involved Institutions


Details

Item typeArticle
Journal or Publication TitleThe Biochemical journal
Publisher:Portland Press
Volume:234
Number of Issue or Book Chapter:1
Page Range:pp. 125-130
Date1986
InstitutionsBiology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz
Identification Number
ValueType
3458463PubMed ID
Classification
NotationType
AnimalsMESH
Calcium/metabolismMESH
Cells, CulturedMESH
DinoprostoneMESH
Enzyme Activation/drug effectsMESH
Glomerular Mesangium/metabolismMESH
MaleMESH
Prostaglandins E/biosynthesisMESH
Protein Kinase C/metabolismMESH
RatsMESH
Rats, Inbred StrainsMESH
Type C Phospholipases/metabolismMESH
Vasoconstrictor Agents/pharmacologyMESH
Verapamil/pharmacologyMESH
Dewey Decimal Classification500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
StatusPublished
RefereedYes, this version has been refereed
Created at the University of RegensburgUnknown
URN of the UB Regensburgurn:nbn:de:bvb:355-epub-270097
Item ID27009

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