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Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source

URN to cite this document:
urn:nbn:de:bvb:355-epub-287907
Kreuzer, Martina ; Schmutzler, Karolin ; Waege, Ingrid ; Thomm, Michael ; Hausner, Winfried
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Date of publication of this fulltext: 04 Sep 2013 06:22


Abstract

BACKGROUND: Bioinformatic analysis of the genes coding for the chitinase in Pyrococcus furiosus and Thermococcus kodakarensis revealed that most likely a one nucleotide insertion in Pyrococcus caused a frame shift in the chitinase gene. This splits the enzyme into two separate genes, PF1233 and PF1234, in comparison to Thermococcus kodakarensis. Furthermore, our attempts to grow the wild type ...

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