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Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source

Kreuzer, Martina, Schmutzler, Karolin, Waege, Ingrid, Thomm, Michael and Hausner, Winfried (2013) Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source. BMC biotechnology 13, p. 9.

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Date of publication of this fulltext: 04 Sep 2013 06:22

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Abstract

BACKGROUND: Bioinformatic analysis of the genes coding for the chitinase in Pyrococcus furiosus and Thermococcus kodakarensis revealed that most likely a one nucleotide insertion in Pyrococcus caused a frame shift in the chitinase gene. This splits the enzyme into two separate genes, PF1233 and PF1234, in comparison to Thermococcus kodakarensis. Furthermore, our attempts to grow the wild type ...

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Item type:Article
Date:2013
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
23391022PubMed ID
10.1186/1472-6750-13-9DOI
Classification:
NotationType
Amino Acid SequenceMESH
Carbon/metabolismMESH
Chitin/metabolismMESH
Chitinase/metabolismMESH
Computational BiologyMESH
Frameshift MutationMESH
Gene Expression/drug effectsMESH
Genetic EngineeringMESH
Hypoxanthine Phosphoribosyltransferase/metabolismMESH
Molecular Sequence DataMESH
Protein Structure, TertiaryMESH
Purines/pharmacologyMESH
Pyrococcus furiosus/metabolismMESH
Sequence AlignmentMESH
Simvastatin/pharmacologyMESH
Thermococcus/enzymologyMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:28790
Owner only: item control page

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