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Simultaneous extraction of nucleic acids and proteins from tissue specimens by ultracentrifugation: A protocol using the high-salt protein fraction for quantitative proteome analysis

Grzendowski, Michael ; Riemenschneider, Markus J. ; Hawranke, Eva ; Stefanski, Anja ; Meyer, Helmut E. ; Reifenberger, Guido ; Stühler, Kai



Abstract

Comprehensive molecular profiling of human tumor tissue specimens at the DNA, mRNA and protein level is often obstructed by a limited amount of available material. Homogenization of frozen tissue samples in guanidine isothiocyanate followed by ultracentrifugation over cesium chloride allows the simultaneous extraction of high-molecular weight DNA and RNA. Here, we present a protocol for ...

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