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Simultaneous extraction of nucleic acids and proteins from tissue specimens by ultracentrifugation: A protocol using the high-salt protein fraction for quantitative proteome analysis

Grzendowski, Michael, Riemenschneider, Markus J., Hawranke, Eva, Stefanski, Anja, Meyer, Helmut E., Reifenberger, Guido and Stühler, Kai (2009) Simultaneous extraction of nucleic acids and proteins from tissue specimens by ultracentrifugation: A protocol using the high-salt protein fraction for quantitative proteome analysis. Proteomics 9 (21), pp. 4985-4990.

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Abstract

Comprehensive molecular profiling of human tumor tissue specimens at the DNA, mRNA and protein level is often obstructed by a limited amount of available material. Homogenization of frozen tissue samples in guanidine isothiocyanate followed by ultracentrifugation over cesium chloride allows the simultaneous extraction of high-molecular weight DNA and RNA. Here, we present a protocol for ...

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Item type:Article
Date:2009
Institutions:Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Hirntumore (ZHT)
Identification Number:
ValueType
19810030PubMed ID
10.1002/pmic.200800902DOI
Classification:
NotationType
Brain Neoplasms/chemistryMESH
Glioma/chemistryMESH
Guanidines/chemistryMESH
HumansMESH
Isothiocyanates/chemistryMESH
Nucleic Acids/isolation & purificationMESH
Proteins/isolation & purificationMESH
Proteome/analysisMESH
ProteomicsMESH
Reproducibility of ResultsMESH
Ultracentrifugation/methodsMESH
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Item ID:29220
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