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Autocrine tumor cell growth-inhibiting activities from human malignant melanoma

Bogdahn, U. ; Apfel, R. ; Hahn, M. ; Gerlach, M. ; Behl, C. ; Hoppe, J. ; Martin, R.


Autocrine-secreted tumor cell growth-inhibiting activities were isolated from supernatants of a malignant melanoma cell line, HTZ 19-dM, established from a central nervous system melanoma metastasis. HTZ 19-dM was characterized by cyto- and immunocytochemistry and karyotyping; cells were propagated in defined serum-free tissue culture medium for up to 8 months. Supernatants were ultrafiltrated, ...


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