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Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae

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Hamperl, Stephan ; Brown, Christopher R. ; Villar Garea, Ana ; Perez-Fernandez, Jorge ; Bruckmann, Astrid ; Huber, Katharina ; Wittner, Manuel ; Babl, Virginia ; Stoeckl, Ulrike ; Deutzmann, Rainer ; Boeger, Hinrich ; Tschochner, Herbert ; Milkereit, Philipp ; Griesenbeck, Joachim
License: Creative Commons: Attribution 3.0
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Date of publication of this fulltext: 12 Feb 2014 11:36


Chromatin is the template for replication and tran- scription in the eukaryotic nucleus, which needs to be defined in composition and structure before these processes can be fully understood. We report an isolation protocol for the targeted purifi- cation of specific genomic regions in their native chromatin context from Saccharomyces cerevisiae . Subdomains of the multicopy ribosomal DNA ...


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