The RNA polymerase trigger loop functions in all three phases of the transcription cycle

URN to cite this document:: urn:nbn:de:bvb:355-epub-295412
DOI to cite this document:: 10.5283/epub.29541

Fouqueau, Thomas ; Zeller, Mirijam Elisabeth ; Cheung, Alan C. ; Cramer, Patrick ; Thomm, Michael

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Date of publication of this fulltext: 12 Feb 2014 12:59

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Abstract

The trigger loop (TL) forms a conserved element in the RNA polymerase active centre that functions in the elongation phase of transcription. Here, we show that the TL also functions in transcription initiation and termination. Using recombinant variants of RNA polymerase from Pyrococcus furiosus and a reconstituted transcription system, we demonstrate that the TL is essential for initial RNA ...