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Absorption and emission spectroscopic characterization of photo-dynamics of photoactivated adenylyl cyclase mutant bPAC-Y7F of Beggiatoa sp.

Penzkofer, Alfons, Stierl, Manuela, Mathes, Tilo and Hegemann, Peter (2014) Absorption and emission spectroscopic characterization of photo-dynamics of photoactivated adenylyl cyclase mutant bPAC-Y7F of Beggiatoa sp. J. Photochem. Photobiol. B: Biology 140, pp. 182-193.

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Abstract

The photoactivated cyclase bPAC of the microbial mats bacterium Beggiatoa sp. consists of a BLUF domain and an adenylyl cyclase domain. It has strong activity of photo-induced cyclic adenylyl monophosphate (cAMP) formation and is therefore an important optogenetic tool in neuroscience applications. The SUMO-bPAC-Y7F mutant where Tyr-7 is replaced by Phe-7 in the BLUF domain has lost the typical ...

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Item type:Article
Date:2014
Institutions:Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer
Identification Number:
ValueType
10.1016/j.jphotobiol.2014.06.017DOI
Keywords:photoactivated cyclase bPAC; BLUF domain; soil bacterium Beggiatoa sp.; triplet based flavin semiquinone photo-cycle; flavin-C4a-adduct photo-cycle; anionic flavin hydoquinone charge transfer complex formation
Dewey Decimal Classification:500 Science > 530 Physics
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:30848
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