Substantial biases in ultra-short read data sets from high-throughput DNA sequencing.

URN to cite this document:: urn:nbn:de:bvb:355-epub-329597
DOI to cite this document:: 10.5283/epub.32959

Dohm, Juliane C. ; Lottaz, Claudio ; Borodina, Tatiana ; Himmelbauer, Heinz

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Date of publication of this fulltext: 03 Dec 2015 10:05

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Abstract

Novel sequencing technologies permit the rapid production of large sequence data sets. These technologies are likely to revolutionize genetics and biomedical research, but a thorough characterization of the ultra-short read output is necessary. We generated and analyzed two Illumina 1G ultra-short read data sets, i.e. 2.8 million 27mer reads from a Beta vulgaris genomic clone and 12.3 million ...