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Dual-fluorescence pH probe for bio-labelling

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Richter, C. ; Schneider, C. ; Quick, M. T. ; Volz, P. ; Mahrwald, R. ; Hughes, J. ; Dick, Bernhard ; Alexiev, U. ; Ernsting, N. P.
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Date of publication of this fulltext: 08 Feb 2016 14:07


Although seminaphtorhodafluor (SNARF) dyes are already widely used to measure pH in cells and at biofilms, their synthesis has low yield and results in an unspecific position of a carboxy-group. The separation of 5′- and 6′-carboxy-SNARF reveals a pKa difference of 0.15, calling into question pH measurements with the (commercially available) mixture. Here we replace the bulky external ...


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