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Characterization of a cosmid library from flow-sorted chromosomes 11

Weber, Bernhard H. F. ; Stöhr, H. ; Siedlaczck, I. ; Longmire, J. L. ; Deaven, L. L. ; Duncan, A. M. V. ; Riess, O.



Abstract

A cosmid library specific for human chromosome 11 has been constructed from flow-sorted chromosomes. The flow-purified chromosomes were prepared from the hamster/human hybrid line J1 which contains chromosome 11 as the only human chromosome. Individual clones were sampled in 187 microtitre plates, resulting in a total of 17 952 colonies. Hybridization analysis revealed that 83.7% of these clones ...

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