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Total phenolic and tannins determination: a modification of Ph. Eur. 2.8.14 for higher throughput

Wiesneth, Stefan ; Jürgenliemk, Guido


A principle mostly being used to quantify phenols and tannins is the Folin-Ciocalteu's reagent method described in the European Pharmacopeia's method 2.8.14. Aim of the present study was to simplify this procedure in order to handle larger sample numbers. By changing the wavelength, the amounts of reagents, miniaturizing the setting to microtiter scale and changing the incubation time before ...


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