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Cobalt labelling of single primary auditory neurones: An alternative to HRP

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Köppl, Christine ; Gleich, Otto
Date of publication of this fulltext: 05 Aug 2009 13:43


We have labelled single, primary auditory neurones in three reptile and one bird species. After functional characterization of the neurones, hexamminecobaltic chloride was iontophoretically injected through the recording micropipette. Precipitation of cobalt sulfide followed by silver intensification of the cochlear duct as a whole-mount preparation revealed stained neurones in over 90% of cases. This method has several advantages over labelling with HRP.

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