"Bligh and Dyer" and folch methods for solid-​liquid-​liquid extraction of lipids from microorganisms. Comprehension of solvatation mechanisms and towards substitution with alternative solvents

URN to cite this document:

urn:nbn:de:bvb:355-epub-377234 Copy

DOI to cite this document:

10.5283/epub.37723 Copy

Breil, Cassandra ; Vian, Maryline Abert ; Zemb, Thomas ; Kunz, Werner ; Chemat, Farid

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Date of publication of this fulltext: 18 Sep 2018 11:55

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Details

Item type:	Article
Journal or Publication Title:	International Journal of Molecular Sciences
Publisher:	MDPI AG
Place of Publication:	BASEL
Volume:	18
Number of Issue or Book Chapter:	4
Page Range:	pp. 708-728
Date:	2017
Institutions:	Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz
Identification Number:	Value Type 10.3390/ijms18040708 DOI
Keywords:	BIO-BASED SOLVENTS; CELL-WALL; SACCHAROMYCES-CEREVISIAE; RAPID METHOD; COSMO-RS; CELLULOSIC BIOETHANOL; YEAST; MICROWAVE; PURIFICATION; ULTRASOUND; Bligh and Dyer; Folch; bio-sourced solvent; yeast; lipids
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	37723

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Abstract

Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being ...

Abstract

Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being potentially suitable for the substitution of methanol and chloroform. We confirm this by performing solid-liquid extraction of yeast (Yarrowia lipolytica IFP29) and subsequent liquid-liquid partition-the two steps of routine extraction. For this purpose, we consider similar points in the ternary phase diagrams of water/methanol/chloroform and water/ethanol/ethyl acetate, both in the monophasic mixtures and in the liquid-liquid miscibility gap. Based on high performance thin-layer chromatography (HPTLC) to obtain the distribution of lipids classes, and gas chromatography coupled with a flame ionisation detector (GC/FID) to obtain fatty acid profiles, this greener solvents pair is found to be almost as effective as the classic methanol-chloroform couple in terms of efficiency and selectivity of lipids and non-lipid material. Moreover, using these bio-sourced solvents as an alternative system is shown to be as effective as the classical system in terms of the yield of lipids extracted from microorganism tissues, independently of their apparent hydrophilicity.

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Metadata last modified: 25 Nov 2020 19:13

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