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"Bligh and Dyer" and folch methods for solid-​liquid-​liquid extraction of lipids from microorganisms. Comprehension of solvatation mechanisms and towards substitution with alternative solvents

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Breil, Cassandra ; Vian, Maryline Abert ; Zemb, Thomas ; Kunz, Werner ; Chemat, Farid
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Date of publication of this fulltext: 18 Sep 2018 11:55


Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being ...


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