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Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells

Jakob, Leonhard, Gust, Alexander and Grohmann, Dina (2018) Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells. Biochemistry and Biophysics Reports 2019 (17), pp. 1-9.

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Date of publication of this fulltext: 23 Jan 2019 18:25

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Other URL: http://doi.org/10.1016/j.bbrep.2018.10.011, https://www.sciencedirect.com/science/article/pii/S2405580818301729?via%3Dihub


Abstract

Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules. Site-specific labelling of a protein is of particular interest for fluorescence-based single-molecule measurements including single-molecule FRET or super-resolution microscopy. The labelling procedure should be highly specific but ...

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Item type:Article
Date:6 November 2018
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Dina Grohmann
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.1016/j.bbrep.2018.10.011DOI
Keywords:Bioorthogonal chemistry; Unnatural amino acids; Amber suppression; eGFP; Genetic code expansion
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:38257
Owner only: item control page

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