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Time course of chemotaxis and chemokinesis of neutrophils following stimulation with IL-8 or FMLP

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Hattenkofer, Matthias ; Gruber, Michael ; Metz, Sophia ; Pfaehler, Sophie-Marie ; Lehle, Karla ; Trabold, Benedikt
Date of publication of this fulltext: 24 Jan 2019 10:30


Polymorphonuclear cells (PMNs) attend to inflammatory sites by chemotactic movement, caused by chemoattractants (CAs) like n-formyl-l-methionyl-l-leucyl-l-phenylalanine (FMLP) and interleukin-8 (IL-8). However, distinct but applicable assays for investigations of PMNs' migration limit in vitro examination. We integrated CD15-bead-based isolation of PMNs with analysing their chemotaxis in a novel ...


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